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af700 conjugated cd31  (R&D Systems)


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    R&D Systems af700 conjugated cd31
    Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), <t>CD31</t> (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+cd31/Human+CD31%2FPECAM-1+Alexa+Fluor%C2%AE+700-conjugated+Antibody/pmc10053540-168-24-26
    Average 93 stars, based on 1 article reviews
    af700 conjugated cd31 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform"

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    Journal: Biofabrication

    doi: 10.1088/1758-5090/ac32a5

    Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), CD31 (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Figure Legend Snippet: Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), CD31 (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Techniques Used: Immunofluorescence, Binding Assay, Construct

    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Figure Legend Snippet: Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Techniques Used: Immunofluorescence, Staining, Construct

    Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.
    Figure Legend Snippet: Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Techniques Used: Construct, Negative Control, Staining



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    af700 conjugated cd31  (R&D Systems)
    93
    R&D Systems af700 conjugated cd31
    Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), <t>CD31</t> (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.
    Af700 Conjugated Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af700+conjugated+cd31/Human+CD31%2FPECAM-1+Alexa+Fluor%C2%AE+700-conjugated+Antibody/pmc10053540-168-24-26
    Average 93 stars, based on 1 article reviews
    af700 conjugated cd31 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), CD31 (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Journal: Biofabrication

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    doi: 10.1088/1758-5090/ac32a5

    Figure Lengend Snippet: Immunofluorescence (IF) of alveolar population at the epithelial layer (a) and the epithelial-endothelial junction (c). (a) maximum projection of alveolar layer only. DAPI (blue), phalloidin (red), caveolin-1/CAV1 (cyan), ATP-binding cassette class A3/ABCA3 (yellow). (b) xyz-view of DAPI (blue), CAV1 (cyan), ABCA3 (yellow) from (a). (c) maximum projection of vascularized alveolar tissue construct (contains both epithelial and vascular layers). DAPI (blue), phalloidin (red), CD31 (cyan), ACE2 (yellow). (d) live perfusion of alveoli-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrow indicatesthe entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Article Snippet: To identify the vascular cells comprising the microvascular network in the gel chamber and the side lanes, FITC-conjugated vimentin (eBioscience TM , 11–9897-82) and AF700-conjugated CD31 (R&D Systems, FAB3567N-100) were used.

    Techniques: Immunofluorescence, Binding Assay, Construct

    Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Journal: Biofabrication

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    doi: 10.1088/1758-5090/ac32a5

    Figure Lengend Snippet: Immunofluorescence (IF) of small airway epithelial population at the epithelial level (a) and at the epithelial-endothelial junction (c), (d). (a) DAPI (blue), α-tubulin (green), MUC5AC (yellow), CK5 (magenta). (b) xyz-view of DAPI (blue), α-tubulin (green), MUC5AC (yellow), and CK5 (magenta) co-stain to show pseudostratified columnar epithelium structure of the small airway layer. (c) maximum projection of epithelial layer only. α-tubulin (green), ACE2 (magenta) along the surface/higher z-stacks of small airway epithelium. (d) maximum projection at vascular layer. DAPI (blue), CD31 (yellow), ACE2 (magenta) along vasculature layer/lower z-stacks of small airway-capillary constructs. (e) live perfusion of small airway-capillary construct at timepoint t=0 min. The dye was introduced into the reservoirs of the left parent channel (input channel/vessel) and the white arrows indicate the entry point from the vascular network into the output channel/vessel. Taken with Opera Phenix. Scale bar = 100 μm unless otherwise indicated in images.

    Article Snippet: To identify the vascular cells comprising the microvascular network in the gel chamber and the side lanes, FITC-conjugated vimentin (eBioscience TM , 11–9897-82) and AF700-conjugated CD31 (R&D Systems, FAB3567N-100) were used.

    Techniques: Immunofluorescence, Staining, Construct

    Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Journal: Biofabrication

    Article Title: Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform

    doi: 10.1088/1758-5090/ac32a5

    Figure Lengend Snippet: Flow cytometric evaluation of cell types in 3D respiratory tissue construct and table with cluster frequencies. (a) bivariate plot of AQP5 and Sp-C in 3D alveoli-capillary construct. (b) bivariate plot of AQP5 and Sp-C on transwell platform, alveolar monoculture. (c) bivariate plot of AQP5 and Sp-C in submerged T-75 alveolar monoculture. (d) bivariate plot of a-tubulin and MUC5AC in 3D small airway-capillary construct. (e) bivariate plot of α-tubulin and MUC5AC on transwell platform, small airway monoculture. (f) bivariate plot of α-tubulin and MUC5AC in submerged, T-75 small airway culture. Grey = negative control; pink – stained sample. (g) bivariate plot of CD31 and vimentin in 3D respiratory construct. (h) bivariate plot of CD31 and vimentin of submerged T-75 monoculture for endothelial cells, fibroblasts and pericyte, contour plots of cell types overlaid. (i) Stacked column graph of cell populations from (a)-(f). VAMC = vascularized alveolar multi-chip; VBMC = vascularized bronchiolar multi-chip; AEC = alveolar epithelial cells; SAEC = small airway epithelial cells; TW = transwell. Blue = endothelial cells; red = fibroblasts; black = pericytes. FlowJo for compensation matrix calculation and gating analysis.

    Article Snippet: To identify the vascular cells comprising the microvascular network in the gel chamber and the side lanes, FITC-conjugated vimentin (eBioscience TM , 11–9897-82) and AF700-conjugated CD31 (R&D Systems, FAB3567N-100) were used.

    Techniques: Construct, Negative Control, Staining